BRIEF REPORT Rapid Differentiation of Polypeptide Chains of Hemoglobin by Cellulose Acetate Electrophoresis of Hemolylsates
نویسنده
چکیده
I DENTIFICATION of the abnormal polypeptide chain of a hemoglobin ( Hb ) variant is often useful in connection with structural analyses and Chernoff and Pettit’ have recently improved on existing methods for this purpose by using starch gel electrophoresis of globin in urea barbital buffer. Izzo2 used this same buffer for electrophoresis of globin on cellulose acetate. Ohba et al.3 have proposed starch gel electrophoresis of whole hemolysates in the presence of p-chloromercuribenzoic acid. We now report a simple, rapid method of differentiating polypeptide chains of hemoglobin by cellulose acetate electrophoresis of whole hemolysates in urea barbital buffer.
منابع مشابه
Rapid differentiation of polypeptide chains of hemoglobin by cellulose acetate electrophoresis of hemolylsates.
I DENTIFICATION of the abnormal polypeptide chain of a hemoglobin ( Hb ) variant is often useful in connection with structural analyses and Chernoff and Pettit’ have recently improved on existing methods for this purpose by using starch gel electrophoresis of globin in urea barbital buffer. Izzo2 used this same buffer for electrophoresis of globin on cellulose acetate. Ohba et al.3 have propose...
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